Part:BBa_K4440001:Design

preproSP-4B4-AGA2-Tyr1-3HA

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The fusion protein should have been in one open reading frame without shift and the tyrosinase sequence was optimized for S. cerevisiae. Also illegal restriction sites for Biobrick compatability were removed

Source

4B4 - Ballard et al., 2011, doi: 10.1089/cbr.2011.0954; preproSP and AGA2 - yeast genome; Tyr1 - Bacillus Megaterium (Xiaolin Shen et. al, 2019, DOI: 10.1038/s41598-019-43577-w); 3HA - Janke C et. al, 2004, DOI: 10.1002/yea.1142